Fig. 1
- ID
- ZDB-FIG-130117-48
- Publication
- Chu et al., 2012 - Yolk syncytial layer formation is a failure of cytokinesis mediated by Rock1 function in the early zebrafish embryo
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Dynamics of yolk cortical microtubules and marginal blastomere boundaries during YSL formation. Whole-mount embryos were stained for β-tubulin (green), F-actin (red) and DNA (blue). All images are projections of multiple focal planes. Insets show higher magnifications of boxed areas in the respective panels. (A) The furrow microtubule array (FMA) between blastomeres (Aiii, arrowhead) is apparent at the 32-cell stage. (B) YCL microtubules of the marginal blastomeres at 128-cell stage. (C) 256-cell stage microtubule arrays extend into the YCL (Ciii inset, curly bracket). Arrowheads in Ai–Ci indicate prominent lower borders between marginal blastomeres. (D) Marginal blastomeres flattened at the 512-cell stage; some have lost cell borders (Di, arrow) while others are still present (Di, arrowheads). Arrow in Diii inset indicates a cell in interphase; arrowhead indicates a mitotic cell. (E) At 1k-cell stage, some marginal cell borders remain (Ei, arrowheads). Arrowheads in Eii indicate large interdigitating aster-like microtubule arrays. (F) At oblong stage, YSN have multiplied and spread vegetally (Fi, arrowhead). Dashed circle in Fiii highlights the microtubule arrays radiating from a vegetal YSN. |
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| Stage Range: | 64-cell to Prim-25 |