FIGURE

Fig. S2

ID

ZDB-FIG-130117-12

Publication

Lagendijk et al., 2012 - Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

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Fig. S2

Specific miRNA expression in EDC fixed embryos which can be complemented with immunohistochemistry. (a,b) MiR-23 expression at 2 dpf in PFA+EDC fixed embryos. (a) Upon injection of a miR-23 MO, miR-23 expression was lost, while highly specific expression (arrowhead in b indicating the endocardial cushions) was detectable in non-injected control embryos (b). (c) Transversal section of an embryonic heart at 3 dpf fixed in PFA+EDC probed for miR-23 (arrowheads point to endocardial cushions) complemented with immunohistochemistry to detect tropomyosin (brown) in the myocardium. Scale bar represents 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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