ZFIN Image: Lagendijk et al., 2012, Fig. S2

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Figure Caption

Fig. S2 Specific miRNA expression in EDC fixed embryos which can be complemented with immunohistochemistry. (a,b) MiR-23 expression at 2 dpf in PFA+EDC fixed embryos. (a) Upon injection of a miR-23 MO, miR-23 expression was lost, while highly specific expression (arrowhead in b indicating the endocardial cushions) was detectable in non-injected control embryos (b). (c) Transversal section of an embryonic heart at 3 dpf fixed in PFA+EDC probed for miR-23 (arrowheads point to endocardial cushions) complemented with immunohistochemistry to detect tropomyosin (brown) in the myocardium. Scale bar represents 50 μm.

Acknowledgments

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