FIGURE

Fig. 2

ID

ZDB-FIG-130115-12

Publication

Mellman et al., 2012 - Fibrillin-2b regulates endocardial morphogenesis in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Heart vasculature defects in sco^te382 mutants. Fluorescence images of 72 hpf Tg(kdrl:GFP)^s843 wild-type (A) and sco^te382 mutant (B) larvae. The overall patterning of the vasculature appears unaffected in sco^te382 mutant larvae; however, pronounced pericardial edema (black arrow in panel A) indicates a severe defect in heart function. Closer inspection reveals the absence of endocardial cells in the mutant atrium (white arrow in panel B). SPIM images show Tg(kdrl:GFP)^s843; Tg(my17:DsRed)^s879 wild-type endocardium and myocardium (C) at 48 hpf and the Tg(kdrl:GFP)^s843sco^te382 mutant atrium, which appears to lack its endocardial lining (D) at 72 hpf. Panels C and D are stills taken from Video 1 and Video 2, respectively. Panels E–G are DIC images of a sco^te382 mutant atrium taken at 40×. An endocardial cell (white arrow) fails to adhere to its neighbor, thereby creating a gap (white arrowhead) in the endocardial sheet; DIC (E), the endothelial kdrl:GFP reporter (F) and merged image (G). A: atrium; V: ventricle.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbn2b^{te382a/te382a}; pku300^{te382b/te382b} fbn2b^te382a; pku300^te382b; s843Tg
Observed In:	atrium cardiac jelly endocardium cell pericardium
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 372(1), Mellman, K., Huisken, J., Dinsmore, C., Hoppe, C., and Stainier, D.Y., Fibrillin-2b regulates endocardial morphogenesis in zebrafish, 111-119, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.