FIGURE

Fig. 9

ID

ZDB-FIG-121101-27

Publication

Cox et al., 2012 - An essential role of variant histone h3.3 for ectomesenchyme potential of the cranial neural crest

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Fig. 9

Cell death in h3f3a^db1092 embryos.

a–c, Lysotracker Red staining marks similar amounts of dying cells in wild-type (n = 2), h3f3a^db1092/+ heterozygous (n = 5), and h3f3a^{db1092/db1092} homozygous (n = 3) embryos at 12.5 hpf. The bright staining in the bottom of each panel is the yolk. d–f, At 16 hpf, increased Lysotracker Red staining (arrows) was evident in the dorsal neural tube of h3f3a^db1092/+ heterozygotes (2/2) and h3f3a^{db1092/db1092} homozygotes (3/3) but not wild types (0/4). These dying cells were located in a similar position to where CNC forms in wild-type embryos. Scale bar = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	h3f3a^{db1092/db1092} h3f3a^db1092/+
Observed In:	neural tube dorsal region
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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