Cdk5, dyrk2, dpysl2, and dpysl3 were involved in the positioning of neural crest cells during neurulation of the spinal cord. (A–R) The expression of sox10 and crestin in the anterior part of the dorsal spinal cord in wild-type embryos (A–F), the dpysl2 and dpysl3 double morphants (G–L), and the cdk5 and dyrk2 double morphants (M–R) at 8- to 18-somite stages. In wild-type embryos, the sox10-positive or the crestin-positive NCCs distributed over the entire dorsal part of the spinal cord through the 8- to 18-somite stages (A–F). In the dpysl2 and dpysl3 double morphants, the sox10-positive or the crestin-positive NCCs also distributed throughout the dorsal part of the spinal cord at the 8-somite stage (G, H). However, at the 14- and 18-somite stages, the number of NCCs was reduced in the medial part of the spinal cord (I–L, surrounded by yellow line). Similarly, in the cdk5 and dyrk2 double morphants, a significant reduction in the number of NCCs was also observed in the medial part of the spinal cord (O–R, surrounded by yellow line). (S–X) Distribution of SOX-10-positive NCCs (arrow heads) along the axons of caudal primary motor neurons (CaP) in the wild-type embryos (S, V), the dpysl2 and dpysl3 double morphants (T, W), and the cdk5 and dyrk2 double morphants (U, X) at 36 hpf.
|
