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Fig. 4

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ZDB-FIG-121026-28

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Tanaka et al., 2012 - Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish

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Fig. 4

The abnormal position of Rohon-Beard neurons caused by loss of function of cdk5 and dyrk2 was rescued by phosphorylation-mimic forms of Dpysl2 and Dpysl3. (A–F) The position of RB neurons in the cdk5 and dyrk2 double morphants which co-injected with normal AMO-resistant (5-mis) dpysl2 or dpysl3 mRNAs (A, D) or with phosphorylation-mimic forms of dpysl2 or dpysl3 mRNAs (B, C, E, F) at 28 hpf. Dorsal view, anterior top. The morphants which co-injected with normal mRNAs showed severe positioning defects in RB neurons (A, D), however, phosphorylation-mimic forms of dpysl2 or dpysl3 mRNAs injected morphants partially recovered their phenotypes (B, C, E, F). (G) Quantitative data for the percentage of RB neurons on the midline. Data are mean ± SEM; ^NP<0.05, ^NNP<0.01, ^NNNP < 0.001, different from the normal mRNA co-injected morphants using 2-tailed paired Student′s t-test.

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Reprinted from Developmental Biology, 370(2), Tanaka, H., Morimura, R., and Ohshima, T., Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish, 223-236, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.