FIGURE

Fig. 4

ID

ZDB-FIG-120717-34

Publication

Oehlers et al., 2012 - Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis

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Fig. 4

DSS-induced mucus secretion protects against further chemical-induced enterocolitis. (A) Schematic describing the experimental procedure analyzed in panels B and C. (B) Live imaging of neutrophil distribution in Tg(mpx:EGFP)ⁱ¹¹⁴ larvae co-treated as indicated. Scale bar: 1 mm. (C) Enumeration of intestinal neutrophils (n≥22 per group; two biological replicates). Error bars indicate s.e.m.; ***P<0.0001 as determined by ANOVA. (D) Schematic describing the experimental procedure reported in panels E and F. (E) Survival analysis of larvae treated as indicated from 3 dpf and exposed to 75 μg/ml TNBS from 6 dpf (n≥10 per group; six biological replicates). Error bars indicate 95% confidence interval. DSS vs control, DSS vs dextran: P<0.0001 as determined by log-rank test. (F) Enumeration of intestinal neutrophils in larvae exposed to 75 μg/ml TNBS from 6 dpf and assayed at 24 hours post-TNBS exposure (n≥12 per group; three biological replicates). Error bars indicate s.e.m.; ***P<0.0001, **P<0.01 as determined by ANOVA.

Expression Data

Gene:	GFP
Fish:	i114Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	neutrophil
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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