aldh1a2 expression domains in larval and adult zebrafish. Dynamic aldh1a2 expression is visible in the mineralizing vertebral column (illustrated by alizarin red staining) as a sheath covering the notochordal surface of larvae at 4.8 mm standard length at different stages of development (A, B). Stable expression was detected in the lamellae of the adult olfactory rosette (olfactory epithelium) (C, D), and in stitches of the anterior and posterior lateral line (E, E′, postoptic region and opercle). A vertical row of six neuromasts located on the dorsal head (F), in which aldh1a2:gfp-positive mantle cells surround the 4-Di-2-ASP-labelled neuromast hair cells (H); enhanced contrast visualizes accompanying nerves (G). In 4.8-mm-sized (standard length) larvae and adults, aldh1a2-positive cells are detected in the corpuscles of stannius (cs) and the collecting ducts (I–K′), as well as in the mature ovaries (ov) and testes (te) (L–M′). All images are oriented with rostral to the left; A, B, E–I′, L–M′: lateral views; C, D: dorsal views; K, K′: ventral views. Scale bars = 100 μm (A, B, D, F–I,), 1 mm (C, E, J–M). cd, collecting duct; li, liver; mc, mantle cells; n, nerves; nc, notochord; nm, neuromasts; op, opercle; or, olfactory rosette; pr, proctodeum/cloaca; sb, swim bladder; v, vertebrae. Fluorescent images of brightfield counterparts are labeled with inverted commas.
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