FIGURE

Fig. S1

ID

ZDB-FIG-120525-37

Publication

Jia et al., 2012 - Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Effectiveness of ppp4ca/b morpholinos, related to Figure 1. In order to test the efficacy of ppp4ca-MO1, ppp4ca-MO2, ppp4cb-MO1 or ppp4cb-MO2, the p4ca-5′UTR-GFP and p4cb-5′UTR-GFP constructs were generated. The 5′UTR containing targeting sequences of MOs and a portion of the coding sequence of ppp4ca or ppp4cb were amplified and inserted in frame to pEGFP-N3 (Clontech) with HindIII and KpnI sites. The plasmid DNA was injected alone or co-injected with each morpholino into one-cell embryos. The injected embryos were observed for GFP expression at the late gastrulation stage. Note that GFP expression was inhibited by specific morpholinos (C, D, G, H) but not by control morpholinos (B, F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 22(5), Jia, S., Dai, F., Wu, D., Lin, X., Xing, C., Xue, Y., Wang, Y., Xiao, M., Wu, W., Feng, X.H., and Meng, A., Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos, 1065-1078, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell