ZFIN Image: Jia et al., 2012, Fig. S1

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Figure Caption

Fig. S1

Effectiveness of ppp4ca/b morpholinos, related to Figure 1. In order to test the efficacy of ppp4ca-MO1, ppp4ca-MO2, ppp4cb-MO1 or ppp4cb-MO2, the p4ca-5′UTR-GFP and p4cb-5′UTR-GFP constructs were generated. The 5′UTR containing targeting sequences of MOs and a portion of the coding sequence of ppp4ca or ppp4cb were amplified and inserted in frame to pEGFP-N3 (Clontech) with HindIII and KpnI sites. The plasmid DNA was injected alone or co-injected with each morpholino into one-cell embryos. The injected embryos were observed for GFP expression at the late gastrulation stage. Note that GFP expression was inhibited by specific morpholinos (C, D, G, H) but not by control morpholinos (B, F).

Acknowledgments

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Reprinted from Developmental Cell, 22(5), Jia, S., Dai, F., Wu, D., Lin, X., Xing, C., Xue, Y., Wang, Y., Xiao, M., Wu, W., Feng, X.H., and Meng, A., Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos, 1065-1078, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell