FIGURE

Fig. 1

ID

ZDB-FIG-120423-22

Publication

Arkhipova et al., 2012 - Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish

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Fig. 1

Motoneuron and beta-cell specific GFP expression in Tg[hb9:GFP]^ml2 embryos. (A) Schematic representation of the hb9 gene from different species. Highlighted are conserved non-coding regions (dark green), and exons (coding sequences in grey, untranslated regions in blue). (B–I2) In situ hybridization analysis comparing mRNA expression of hb9 (B, D, D2) and gfp (C, E, E2) in Tg[hb9:GFP]^ml2 embryos at 17 hpf (B, C) and 24 hpf (D, E) reveals similar pattern in motoneurons and ventral interneurons (B–E, white arrows) and pancreas (B, C, white arrowheads). Asterisk in C marks ectopic gfp expression in rhombomere. D2, E2 show different focal planes of the embryos shown in D, E. (F–I) Confocal image projections of Tg[hb9:GFP]^ml2 embryos labelled with GFP (green) and islet antibodies (red) at 16 hpf (F), 19 hpf (G) and 24 hpf (H) show overlapping expression of GFP and islet proteins in motoneurons (arrows) and in a subset of endocrine cells (arrowheads). (H–I2) Confocal image stacks of Tg[hb9:GFP]^ml2 embryos labelled with antibodies for GFP and pancreatic hormones at 24 hpf (H2) and 48 hpf (I, I2) show that GFP overlaps with insulin (blue in H2, I) but not with glucagon (Glu) and somatostatin (Sst) (Glu + Sst in red, I2). Embryos are shown in dorsal (B–E) or ventral (F–I) view with anterior to the left. Scale bars in all figures correspond to 50 μm.

Expression Data

Genes:	GFP mnx1
Antibodies:	Ab2-gcg Ab2-isl Ab3-GFP Ab3-ins Ab3-sst
Fish:	AB ml2Tg
Anatomical Terms:	endocrine cell interneuron motor neuron pancreas primordium pancreatic A cell pancreatic B cell pancreatic bud pancreatic D cell
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 365(1), Arkhipova, V., Wendik, B., Devos, N., Ek, O., Peers, B., and Meyer, D., Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish, 290-302, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.