FIGURE

Fig. S2

ID

ZDB-FIG-120416-6

Publication

Ninov et al., 2012 - Different levels of Notch signaling regulate quiescence, renewal and differentiation in pancreatic endocrine progenitors

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Analysis of Tg(Tp1:VenusPest) expression after a brief period of Notch signaling downregulation. Tg(Tp1:H2BmCherry); Tg(Tp1:VenusPest) larvae were treated with DMSO or LY411575 from 4 to 4.5 dpf. (A) In DMSO-treated larvae, the vast majority of pancreatic NRCs were Tg(Tp1:H2BmCherry)⁺ and Tg(Tp1:VenusPest)⁺. (B,B2) Larvae treated with 10 μM LY411575 showed variable donwregulation of Tg(Tp1:VenusPest) expression. (B) Seven out of 10 larvae (Class1) showed minor downregulation of Tg(Tp1:VenusPest) expression. In these larvae, only some Tg(Tp1:H2BmCherry)⁺ were Tg(Tp1:VenusPest)^- (white arrowheads). (B2) Three out of 10 larvae (Class 2) exhibited significant downregulation of Tg(Tp1:VenusPest) expression and multiple Tg(Tp1:H2BmCherry)⁺ were Tg(Tp1:VenusPest)^- compared with DMSO controls (white arrowheads). (C) Larvae treated with 50 μM LY411575 showed consistent downregulation of Tg(Tp1:VenusPest) expression compared with DMSO controls. In these larvae, many Tg(Tp1:H2BmCherry)⁺ cells were Tg(Tp1:VenusPest)^- (white arrowheads). All images are lateral views, anterior towards the top, ventral towards the right; white arrowheads indicate the IPD. The NRCs ventral to the IPD are gut cells. Scale bars: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development