FIGURE

Fig. S7

ID

ZDB-FIG-120405-28

Publication

Jao et al., 2012 - A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization

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Fig. S7

The distribution of cleaved caspase 3⁺ cells is mutually exclusive with that of differentiated neurons in Gle1-deficient embryos. (A-I) Transverse cryosections through the spinal cords of gle1 morphants were co-stained with anti-Elavl and anti-cleaved caspase 3 antibodies at 24 hpf. The cleaved caspase 3⁺ cells (B,E,H) are distributed widely along the dorsoventral axis of the spinal cord, often located close to the central canal or immediately adjacent to the basally located Elavl⁺ neurons (A,D,G). However, cleaved caspase 3⁺ and Elavl⁺ signals do not overlap (merged channels, C,F,I). Each image is a single optical section (<1 µm) extracted from the confocal z-stack with dorsal upward. Dashed lines outline spinal cords and central canals. Scale bar: 10 µm. (J) Distribution of the cleaved caspase 3⁺ cells in the transverse sections of the gle1 morphant spinal cords at 24, 36 and 52 hpf. Single confocal sections were manually superimposed and the positions of the cleaved caspase 3⁺ cells were marked. The cleaved caspase 3⁺ cells are distributed widely along both the dorsoventral and apical-basal axes of the spinal cord.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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