Motoneuron phenotypes of gle1 morphants. (A-F) Confocal images of live control (A,D) and gle1 morphant (B,C,E,F) zebrafish embryos with motoneurons labeled by mnx1:TagRFP-T transgene at 50 hpf (A-C) and 82 hpf (D-F). gle1 morphants were injected with buffer alone (B,E) or with <140 pg in vitro transcribed EGFP-tagged human GLE1b mRNA (C,F). gle1 morphants lack rostrally projecting nerves and show pronounced defects in motor axon arborization (B,E), especially at 82 hpf, when the gle1 morphant also lacks dorsally projecting axons (E, brackets) and aberrations in ventrally projecting axons are particularly evident. Expressing human GLE1 efficiently rescues the arborization defects (C,F). Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bars: 50 μm.
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