FIGURE

Fig. S3

ID

ZDB-FIG-120315-73

Publication

Schaub et al., 2012 - Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network

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Fig. S3

Phenocopy of the s445 mutant phenotype by morpholino antisense oligonucleotides. (A–F) Lateral views of wild-type control (A and B), snapc4 MO(A) injected (C and D), and snapc4 MO(B) injected (E and F) larvae at 120 hpf. Tg(ela3l:EGFP)^gz15 expression is shown in B, D, and F. snapc4 MO(A) injected larvae show an identical phenotype to that of s445 mutant larvae. Lateral views, anterior to the left (A, C and E) or the right (B, D and F). (G) Schematic of snapc4 morpholino target locations. MO(A) is designed to target the splice junction prior to the Snapc2 binding domain. MO(B) is designed to target the splice junction prior to the Myb DNA binding domain. MO(C) is designed to target the translation initiation site. (H) Reverse transcriptase-PCR analysis shows that snapc4 mRNA was truncated in snapc4 MO(B) injected larvae.

Expression Data

Gene:	EGFP
Fish:	gz15Tg gz15Tg + MO1-snapc4 gz15Tg + MO2-snapc4
Knockdown Reagents:	MO1-snapc4 MO2-snapc4
Anatomical Term:	gut
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-snapc4 WT + MO2-snapc4
Knockdown Reagents:	MO1-snapc4 MO2-snapc4
Observed In:	liver liver and biliary system
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(1), Schaub, M., Nussbaum, J., Verkade, H., Ober, E.A., Stainier, D.Y., and Sakaguchi, T.F., Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network, 128-137, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.