FIGURE

Fig. 4

ID

ZDB-FIG-120315-68

Publication

Schaub et al., 2012 - Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network

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Fig. 4

Positional cloning of the s445 mutant locus. (A) Schematic of the 445 locus. s445 is located on chromosome 5 in between two CA-repeat markers, z26603 (0.2 cM; 7 recombinants out of 3072 meioses) and CA-YE2 (0.07 cM; 2 recombinants out of 3056 meioses). The critical region of the s445 locus was spanned by two partially overlapping BAC clones, DKEY-20G15 and DKEY-1O2. (B) A single nucleotide mutation (C to T) that results in a premature stop of the snapc4 gene was identified in s445 mutants (0 recombinants out of > 3000 meioses). (C) Schematic of zebrafish Snapc4. Zebrafish Snapc4 contains a conserved Myb DNA binding domain and a Snapc2 binding domain. The s445 mutation generates a premature stop codon in the zebrafish snapc4 cDNA. The truncated mutant Snapc4 protein is approximately two-thirds the length of wild-type Snapc4 and lacks the Snapc2 binding domain. (D) s445 is located on zebrafish chromosome 5 in a region syntenic to a region of human chromosome 9q34.3. (E) Snapc4 expression in wild-type larvae at 80 hpf. snapc4 expression is detected in the liver at this stage. The outlined area is magnified and shown in E2. Lateral views, anterior to the left. Cross section of the stained larva in the region of the liver is shown in E3. The liver is outlined in E2 and E3.

Expression Data

Gene:	snapc4
Fish:	WT
Anatomical Terms:	brain eye liver pharyngeal arch 3-7
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(1), Schaub, M., Nussbaum, J., Verkade, H., Ober, E.A., Stainier, D.Y., and Sakaguchi, T.F., Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network, 128-137, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.