FIGURE

Fig. 1

ID

ZDB-FIG-120315-10

Publication

Prendergast et al., 2012 - The metalloproteinase inhibitor Reck is essential for zebrafish DRG development

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Fig. 1

sensory-deprived (sdp) mutants exhibit a complete loss of DRG sensory neurons. (A) Tg(neurog1:egfp) zebrafish embryos (3 dpf) immunostained for Elavl1. (A2) DRG are absent in an sdp^w12/w12 mutant embryo. Scale bar: 500 μm. (B,B2) High magnification images of A and A2. Scale bar: 50 μm. (C,C2) neurog1 expression at 48 hpf. Box indicates position of high magnification images in D,D2. Scale bar: 250 μm. (D,D2) High magnification image of C and C2. Scale bar: 50 μm. Arrowheads indicate neurog⁺ DRG sensory neuron precursors. (E,E2) Tg(sox10:eos) embryo (3 dpf) immunostained for Elavl1. Although Schwann glia (empty arrowhead) are retained, satellite glia (filled arrowhead) are absent in sdp^w12/w12. Scale bar: 25 μm. (F,F2) Counts of neurog1⁺ DRG at 3 dpf. Approximately 25% of embryos fail to form DRG, suggesting that sdp^w12 is a fully penetrant recessive mutation. (G) Counts of neurog1⁺ DRG followed over four days. DRG never appear in <25% of the population, indicating that sdp does not delay in DRG development.

Expression Data

Genes:	elavl1a Eos neurog1
Antibody:	Ab1-elavl1
Fish:	AB reck^w12/w12 (AB) w9Tg w61Tg reck^w12/w12; w9Tg reck^w12/w12; w61Tg
Anatomical Terms:	dorsal root ganglion myelinating Schwann cell perineuronal satellite cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	reck^w12/w12 (AB)
Observed In:	dorsal root ganglion
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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