FIGURE

Fig. S2

ID

ZDB-FIG-120201-35

Publication

Cheung et al., 2012 - Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b

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Fig. S2

Generation and validation of Cldn15lb peptide antibody. a, Sequence alignment between zebrafish Cldn10, Cldn15, Cldn15lb-a, Cldn15lb-b, and Cldn10l. [Cldn15lb-a and -b are the proteins encoded by cldn15lb_tv1 and cldn15lb_tv2, respectively.] Purple shading boxes indicate transmembrane regions. Red box highlights the epitope targeted by the peptide antibody. b, HEK293 cells were transiently transfected with constructs expressing Cldn10l, Cldn15, or Cldn15lb-a. Immunofluorescence with peptide antibody generated against Cldn15lb recognized Cldn15lb-a only. c, Cos7 cells were transiently transfected with untagged or GFP-tagged version of either transcript variant. The antiserum recognized cells that were transfected with either untagged transcript variant and it co-localized with the GFP-tagged version. Thus, the polyclonal antibody in the serum recognizes Cldn15lb but does not distinguish between the 2 isoforms.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 361(1), Cheung, I.D., Bagnat, M., Ma, T.P., Datta, A., Evason, K., Moore, J.C., Lawson, N.D., Mostov, K.E., Moens, C.B., and Stainier, D.Y., Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b, 68-78, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.