FIGURE
Fig. S6
- ID
- ZDB-FIG-120201-22
- Publication
- Dworkin et al., 2012 - Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways
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Fig. S6
Generation, characterisation and effects of a spec1 exon 1/intron 1 splice-blocking morpholino. (A) Schematic of PCR-primers used to detect non-spliced reverse-transcribed mRNA product following block of splicing of spec1. (B) A specific spec1 non-spliced product was detectable by RT-PCR; this product was absent in MO:control-injected fish. (C,D) The spec1 splice-blocking MO reproduced the MHB-folding phenotype (C) observed with the spec1 ATG-blocking MO, although less apoptosis was visible (TUNEL; D). |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Prim-5 |
Phenotype Detail
Acknowledgments
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