FIGURE

Fig. 5

ID

ZDB-FIG-120131-35

Publication

Wang et al., 2011 - Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population

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Fig. 5

Expression of dkk1, a Wnt signaling inhibitor, is increased in mob;mos embryos, whereas expression of canonical Wnt targets is suppressed. (A–D) Dorsal view of embryos stained with dkk1 riboprobes in wild-type (WT) (A), mob (B), mos (C), and mob;mos (D) at 75% epiboly (8 hpf). dkk1-positive cells are slightly more in mob or mos, but are highly increased in mob;mos embryos. Insets in A and D show transverse sections through the dkk1-expressing region in the mesendoderm. (E) Quantitative PCR analysis of canonical Wnt signaling targets axin2 and sp5l mRNA levels in wild-type embryos and tfap2a-and foxd3-MO knockdown morphants at 8 hpf. Error bars represent standard deviation. Data analyzed by Student′s t-test, **, P < 0.01 and ***, P < 0.001. (F–I) Morpholino-mediated knockdown of dkk1 partially restores pharyngeal arch formation in mob;mos embryos. Ventral view of Alcian blue-stained heads at 5 dpf showing the craniofacial skeleton of wild-type (F), dkk1 morphants (G), mob;mos (H), and dkk1-MO knockdown mob;mos (I) embryos. Head cartilage in mob;mos embryos injected with dkk1-MO is partially restored (red arrows). (J) Percentage of mob;mos embryos with no pharyngeal skeleton and rescued pharyngeal arches with or without (Control) dkk1-MO injection. n indicates the number of embryos examined, N the number of experiments performed. Data analyzed by Chi-squared test; ***, p < 0.001.

Expression Data

Gene:	dkk1b
Fish:	WT foxd3^m188/m188 tfap2a^m610/m610 tfap2a^m610/m610; foxd3^m188/m188
Anatomical Term:	axial hypoblast
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-dkk1b tfap2a^m610/m610; foxd3^m188/m188 tfap2a^m610/m610; foxd3^m188/m188 + MO2-dkk1b
Knockdown Reagent:	MO2-dkk1b
Observed In:	pharyngeal arch pharyngeal arch cartilage whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.