FIGURE

Fig. 1

ID

ZDB-FIG-120118-21

Publication

Xu et al., 2012 - Gβγ signaling controls the polarization of zebrafish primordial germ cells by regulating Rac activity

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Fig. 1

Gβγ signaling is required for the migration, but not differentiation, of PGCs. Embryos injected with RNAs encoding GFP-nos1-3′UTR, either alone (control, 100 pg) or together with Gαt-nos1-3′UTR (50 pg) or Gβ2C68S-nos1-3′UTR (40 pg). Normally localized (arrow) and ectopic (arrowheads) PGCs are indicated. (A-I) Lateral views of embryos showing PGCs labeled with GFP (A-C), expressing nos1 detected by in situ hybridization (D-F) or labeled with vasa-DsRed and GFP (insets show high-magnification images) (G-I). (J-L) The expression of cxcl12a (outlined with magenta dots) and nos1 by in situ hybridization. Dorsal views. Scale bars: 200 μm. (M) The percentage of embryos with PGC migration defects, with the latter defined as more than three PGCs (detected by nos1 expression) per embryo present outside the presumptive gonad region at 24 hpf (Dumstrei et al., 2004). ^*P<0.01 versus control. (N) Total number of ectopic PGCs per embryo. ^**P<0.01; ^#P>0.5 versus control. Data are mean+s.e.m.

Expression Data

Genes:	cxcl12a nanos1
Fish:	WT
Conditions:	chemical treatment: drug chemical treatment: pharmaceutical chemical treatment: pharmaceutical
Anatomical Terms:	lateral plate mesoderm primordial germ cell
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment: drug chemical treatment: pharmaceutical
Observed In:	germ cell migration primordial germ cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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