FIGURE

Fig. 3

ID

ZDB-FIG-111212-4

Publication

Takeuchi et al., 2007 - Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry

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Fig. 3

Baf60c is sufficient to induce Nodal. Cultured embryos transfected with an EGFP expression construct and Baf60c, activated Notch (NIC), or Nodal expression constructs, or EGFP vector alone (control) were cultured for 15 h. (A, D, G, and I) EGFP fluorescence, showing transfected cells. (A–C) Control studies showed normal/bilateral nodal (B) at the node and Notch1 (C) expression around the node. (D–H) Smarcd3-transfected embryos: ectopic Nodal induction along the midline was seen (E and H), and expansion of Notch1 expression was also observed (F, red arrowhead). (I and J) Overexpression of NIC enhanced Nodal expression. (K) Histogram shows the length (in millimeters) of Nodal expression on the node between the left side (light purple, control) and the right side (light blue, injected experiments, n = 5).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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