ZFIN Image: Takeuchi et al., 2007, Fig. 3

Image

Figure Caption

Fig. 3

Baf60c is sufficient to induce Nodal. Cultured embryos transfected with an EGFP expression construct and Baf60c, activated Notch (NIC), or Nodal expression constructs, or EGFP vector alone (control) were cultured for 15 h. (A, D, G, and I) EGFP fluorescence, showing transfected cells. (A–C) Control studies showed normal/bilateral nodal (B) at the node and Notch1 (C) expression around the node. (D–H) Smarcd3-transfected embryos: ectopic Nodal induction along the midline was seen (E and H), and expansion of Notch1 expression was also observed (F, red arrowhead). (I and J) Overexpression of NIC enhanced Nodal expression. (K) Histogram shows the length (in millimeters) of Nodal expression on the node between the left side (light purple, control) and the right side (light blue, injected experiments, n = 5).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA