Fig. 4

Endogenous Pwp2h in the Gt (pwp2h-citrine)^ct143a line at 31 hours post fertilization (hpf) is localized to proliferative domains in the eye (PCNA [proliferating cell nuclear antigen], red). To enhance the Citrine signal, the sections were stained with anti-green fluorescent protein (GFP) antibody (green). All nuclei are stained with DAPI (42,6-diamidino-2-phenylindole; blue). All sections are transverse cryosections. A: Confocal section showing that most of the Citrine tagged Pwp2h (A–A2) is localized to regions also positive for PCNA (A) in the retina (r, highlighted areas). Some expression is also detected in the lens. Inset, high magnification of the boxed region showing nuclei colocalized with PCNA and Citrine. Arrowheads: Citrine localization to areas that appear to correspond to nucleoli. B: Another example through a different axial level of the eye showing colocalization between PCNA and Citrine (highlighted area). Inset: shows Citrine expression only. Scale bars = 50 μm in A,B, 10 μm in inset in A–A2.