PUBLICATION
Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development
- Authors
- Jayasena, C.S., Trinh, L.A., and Bronner, M.
- ID
- ZDB-PUB-111007-1
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(11): 2578-83 (Journal)
- Registered Authors
- Bronner-Fraser, Marianne, Jayasena, Chathurani (Saku), Trinh, Le
- Keywords
- Pwp2h, zebrafish, ribosome biogenesis, development
- MeSH Terms
-
- Molecular Sequence Data
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Imaging, Three-Dimensional
- Sequence Homology
- Cell Cycle Proteins/genetics*
- Cell Cycle Proteins/metabolism
- Animals, Genetically Modified
- Amino Acid Sequence
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Video Recording/methods
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Cell Tracking/methods
- Embryonic Development/genetics
- Calcium-Binding Proteins/genetics
- Calcium-Binding Proteins/metabolism
- Organic Anion Transporters/genetics
- Organic Anion Transporters/metabolism
- Animals
- PubMed
- 21954116 Full text @ Dev. Dyn.
Citation
Jayasena, C.S., Trinh, L.A., and Bronner, M. (2011) Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(11):2578-83.
Abstract
Yeast Periodic tryptophan protein 2 gene (Pwp2) is involved in ribosome biogenesis and has been implicated in regulation of the cell cycle in yeast. Here, we report a zebrafish protein-trap line that produces fluorescently tagged Periodic tryptophan protein 2 gene homologue (Pwp2h) protein, which can be dynamically tracked in living fish at subcellular resolution. We identified both full-length zebrafish Pwp2h and a short variant. The expression results show that Pwp2h is present in numerous sites in the early developing embryo, but later is restricted to highly proliferative regions, including the forebrain ventricular zone and endoderm-derived organs in the early larval stage. At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis. This Pwp2h protein trap line offers a powerful tool to study the link between ribosome biogenesis and cell cycle progression during vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping