FIGURE

Fig. 3

ID

ZDB-FIG-111007-16

Publication

Kim et al., 2011 - Cug2 is essential for normal mitotic control and CNS development in zebrafish

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Fig. 3

Knock-down analysis of cug2 by morpholinos. A. Genomic structure and MO targeting region (red arrow) of the zebrafish cug2 gene. B. Confirmation of the splice-blocking effect of MO by RT-PCR using p1-p2 primers (in A). Aberrant transcripts were amplified only in cug2 MO-injected embryos. β-actin is the loading control. C-F. In contrast to the control MO (C), cug2 MO-injected embryos (D) display neurodegenerative phenotypes including a flat head, small eyes, pinched midbrain-hindbrain boundary, thin yolk extension, and curved-up body at 3 dpf. Injection of p53 MO (E) did not affect these phenotypes in cug2 morphants, while co-injection of wild-type cug2 mRNA (F) rescued it at 3 dpf. G, H. Detection of cell death in CNS by acridine orange staining of cug2 MO- (H) and control MO-injected (G) embryos at 28 hpf. Scale bars = 250 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-cenpw WT + MO11-tp53 + MO2-cenpw
Knockdown Reagents:	MO2-cenpw MO11-tp53
Observed In:	apoptotic process extension eye head midbrain hindbrain boundary trunk
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.