Fig. S2

Related to Figure 2. obd/+ and WT Embryos Have Identical EC Abundances, but ECs of These Genotypes Distribute Differently within the Trunk Vasculature of WT Hosts

(A) Quantification of EC abundance at 32 hpf in WT (grey bars) and obd/+ (black bars). Abbreviations: Segmental arteries (SeAs), Axial vessels (AxV), Dorsal Longitudinal Anastomotic Vessels (DLAVs). n = 11 WT, n = 13 obd/+. Error bars, s.e.m. (B) Left, example of the trunk vasculature of a 21-23 hpf chimera made using a WT donor and an obd/+ host. ECs by origin: donor (green nuclei), host (red). Somite boundaries, blue. EC nuclei of donor origin found within the host’s aorta abbuting the somite boundary (white arrowhead) or within the central dorsal (CD) region of this vessel (yellow arrowhead) are indicated. White asterisk marks ECs presumably migrating ventrally towards the vein. Top right, diagram of the 21-23 hpf trunkvasculature (red). Somite boundaries (SB), blue. Arterial tree: SeAs and aorta. Aorta subdivided into anterior dorsal (AD), central dorsal (CD), posterior dorsal (PD), somite boundary-abutting (SB), anterior ventral (AV), central ventral (CV) and posterior ventral (PV) regions. Bottom, distribution of ECs of donor origin within the arterial tree of both kinds of WT:obd/+ chimeras. n = 27 each. *p < 0.05, ***p < 0.001. Error bars, s.e.m. (C) Quantification, along with representative images, of the frequency with which donor cells contribute to SeA sprouts in obd-to-WT and WT-to-obd transplants. n = 60 for WT-to-obd, n = 65 for obd-to-WT. (C-D) Confocal images and diagram: Anterior, left; dorsal, up. Scale bars 30 μm.