FIGURE

Fig. 6

ID

ZDB-FIG-110915-14

Publication

Zygmunt et al., 2011 - Semaphorin-PlexinD1 Signaling Limits Angiogenic Potential via the VEGF Decoy Receptor sFlt1

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Fig. 6

Notch Signaling Loss Does Not Phenocopy obd

(A and B) Expression of Notch′s activity nuclear reporter Tg(Tp1bglob:hmgb1-mCherry)^jh11 (red) in the endothelium (gray) of WT (A) and obd (B).

(C–F) obd; mib. (C) Endothelium, green. SBs, red. (D–F) WISH with sflt1, flt4 and mflt1 riboprobes, as indicated. Double mutant phenotypes classed as obd-like (C and D) or mib-like (E and F) based on the mutant they resemble most. Note lack of sflt (as in Figure 3G) and ectopic aortic flt4 (yellow arrowhead; as in Figure S6A) and venous mflt1 stainings (green arrowhead, as in Figure S6B).

(G–I) Angiogenic cell abundance within the trunk′s arterial tree of WT, obd, mib (G) and obd; mib (H) in Tg(fli1:nEGFP)^y7 embryos.

(G–H) EC nuclei, green. SBs, red.

(I) Quantification; n = 10 per genotype.

(J–L) SeA sprout abundance in plcg, mib; plcg, obd; plcg (J) and obd; plcg embryos injected with 10 ng of mib MO (mib MO) (K).

(J and K) Endothelium, green. SBs, red.

(L) n = 8, 7, 11 and 9 for plcg, mib; plcg, obd; plcg and obd; plcg (mib MO), respectively.

Scale bars represent 50 μm (A, B, and D–F), 30 μm (C, G, H, J, and K).

(I and L) ^*p < 0.05, ^***p < 0.001. Error bars represent SEM.

(A–F, G, H, J, and K) Anterior, left; dorsal, up. Trunk images and quantifications: 32 hpf (A–C, G–L), 28 hpf (D–F).

Expression Data

Gene:	mCherry
Fish:	jh11Tg plxnd1^{fov01b/fov01b}; jh11Tg
Anatomical Term:	blood vessel endothelial cell nucleus
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^m178/m178; y7Tg plxnd1^{fov01b/fov01b}; jh11Tg mib1^m178/m178; plxnd1^{fov01b/fov01b}; y7Tg plcg1^y18/y18; plxnd1^{fov01b/fov01b}; y1Tg plcg1^y18/y18; plxnd1^{fov01b/fov01b}; y1Tg + MO1-mib1
Knockdown Reagent:	MO1-mib1
Observed In:	endothelial cell intersegmental artery angiogenic sprout Notch signaling pathway
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 21(2), Zygmunt, T., Gay, C.M., Blondelle, J., Singh, M.K., Flaherty, K.M., Means, P.C., Herwig, L., Krudewig, A., Belting, H.G., Affolter, M., Epstein, J.A., and Torres-Vazquez, J., Semaphorin-PlexinD1 Signaling Limits Angiogenic Potential via the VEGF Decoy Receptor sFlt1, 301-314, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell