Fig. S5

Expression patterns of taste bud related markers in wild-type, control MO, single MOs and triple MOmiR-200-injected embryos. Confocal projections of ventral views, rostral towards the left. Scale bars: 15 μm. (A,B) pH3 (green) expression is comparable between wild-type (A) and triple MOmiR-200-injected (B) embryos, whereas Calb2b expression is severely reduced in the triple MOmiR-200 injected (B) embryo. (C-E2) Comparison of 5HT (green) and Calb2b (red) expression in wild-type (C), control MO (D) and triple MOmiR-200-injected (E) embryos. C and E are lower magnifications of the pictures shown in Fig. 5C,D. Calb2b cells are severely reduced but 5HT cells only mildly (E) compared with the controls in C,D. This reduction is not due to developmental delay (compare with Fig. S1A-C in the supplementary material). C2,E2 are confocal projections of 5HT and Calb2b expression in the brain (hypothalamus, hy; raphe, ra) of the embyos shown in C,E, respectively, showing that brain morphology is comparable between wild-type and triple MOmiR-200-injected embryos. (F,G) Comparison of Calb2b (green) and Col2a (red) expression in wild-type (F) and triple MOmiR-200 injected (G) embryos. Cartilage anatomy (red) is comparable between F and G, whereas Calb2b+ cells are severely reduced in G. (H-J) Single MO injections for miR-200a, miR-200b or miR-429 do not obviously affect taste bud development. Compare embryos injected with MOmiR-200a (H), MOmiR-200b (I), MOmiR-429 (J), with the wild-type embryo shown in Fig. 1A. Statistical analysis of control and MOmiR-200-injected embryos is provided in Table 3.