FIGURE

Fig. 2

ID

ZDB-FIG-110804-11

Publication

Kapsimali et al., 2011 - Fgf signaling controls pharyngeal taste bud formation through miR-200 and Delta-Notch activity

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Fig. 2

Taste bud development requires Fgf signaling. (A) Heatshock and SU5402 treatments in tg(hsp70l:dnfgfr1-EGFP)pd1/+ and wild-type embryos, respectively. (B-I) Ventral views of whole-mounted larvae heads. Asterisks indicate taste buds in the lips. Experimental conditions are indicated in the top right-hand corner, embryonic stage and scale bar are in the bottom left-hand corner and marker expression is in the bottom right-hand corner. Anterior is towards the left. (B-E) Calb2b (B,C, red), 5HT (B,C, cyan) and miR-200a (D,E, blue) expression in control (B,D) and Hsdnfgfr1+ (C,E) larvae after heatshock. (F,G) Wild-type immunostained for Calb2b after DMSO (F) and SU5402 (G) treatment, respectively. (H,I) Wild-type and ace^–/– siblings (severe phenotype, for mild see Fig. S3A-D in the supplementary material) immunostained for Calb2b with strong reduction (arrow) of taste bud cells. Arrowheads in H indicate the palate (p) taste buds. Scale bars: 10 μm. b1-b5, branchial arches 1-5; h, hyoid arch; m, mandibular arch; oe, olfactory epithelium.

Expression Data

Genes:	calb2b mir200a
Antibodies:	Ab1-calb2 Ab1-serotonin
Fish:	WT fgf8a^{ti282a/ti282a} pd1Tg/+
Conditions:	chemical treatment: SU5402 heat shock
Anatomical Terms:	lip epithelium taste bud olfactory epithelium oral epithelium taste bud pharyngeal arch taste bud
Stage Range:	Pec-fin to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT fgf8a^{ti282a/ti282a} pd1Tg/+
Conditions:	chemical treatment: SU5402 heat shock
Observed In:	fibroblast growth factor receptor signaling pathway oral epithelium taste bud taste bud formation
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

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