FIGURE

Fig. 4

ID

ZDB-FIG-110720-8

Publication

Anderson et al., 2011 - The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration

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Fig. 4

tm4sf4 inhibits α and β cell specification, and is necessary for µ cell fates. (A-P) Hormone mRNA levels were measured by qRT-PCR comparing uninjected (blue) and tm4sf4 morphant (red) zebrafish embryos: insulin (A), glucagon (D), ghrelin (K), somatostatin (N). Hormone mRNA expression pattern was determined by in situ hybridization comparing uninjected and tm4sf4 morphant embryos at 20.5 hpf for insulin (B,C) and glucagon (E-J), and at 48 hpf for ghrelin (L,M) and somatostatin (O,P). All images were taken at 40× magnification except G,H taken at 10×; yellow boxes represent areas enlarged in I and J. (Q) Knockdown efficiency of Tm4splMO was determined at all stages. Error bars represent s.e.m. ^*P<0.05.

Expression Data

Genes:	gcga ghrl ins sst1.1
Fish:	WT WT + MO2-tm4sf4
Knockdown Reagent:	MO2-tm4sf4
Anatomical Terms:	pancreatic A cell pancreatic B cell pancreatic epsilon cell somatostatin secreting cell
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-tm4sf4
Knockdown Reagent:	MO2-tm4sf4
Observed In:	endocrine pancreas development pancreatic A cell pancreatic B cell pancreatic epsilon cell
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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