FIGURE

Fig. S3

ID

ZDB-FIG-110720-13

Publication

Anderson et al., 2011 - The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration

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Fig. S3

Tm4atgMO and Tm4splMO induce the same phenotype in a dose-dependent manner and tm4sf4 mRNA rescues the phenotype.30 hpf insulin mRNA expression was visualized by in situ hybridization and fluorescence in situ hybridization (FISH) and was compared between uninjected embryos and embryos injected with 4 ng, 5 ng or 8 ng of either Tm4atgMO or Tm4splMO at 30 hpf. tm4sf4 mRNA (100 pg) was co-injected with 5 ng Tm4splMO. From FISH, insulin⁺ cells were quantified and represented graphically. An earlier insulin phenotype was not seen at 13.5 and 15.5 hpf by qRT-PCR (ten pooled embryos per sample). Error bars represent s.e.m. *P<0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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