FIGURE

Fig. 7

ID

ZDB-FIG-110701-6

Publication

Itou et al., 2011 - HMGB factors are required for posterior digit development through integrating signaling pathway activities

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Downregulation of Wnt signaling and shh signaling in hmgb-knockdown pectoral fin buds. Dorsal views (A–H) and lateral views (I, J) of 36-hpf embryos injected with control MO (A, C, E, G, I) or hmgb1MO + hmgb2 MO (B, D, F, H, J). A, B: Normal expression of prx1 in the pectoral fin bud of control (A, arrows) and hmgb-knockdown embryos (B, arrows). C–H: Expression of Top-GFP (D), shh (F), and ptc1 (H) in the pectoral fin bud of hmgb-knockdown embryos (D, F, H, red arrows) is downregulated, compared to control embryos (C, E, G, black arrows). I, J: High-magnification images of the pectoral fin bud stained with shh shows smaller expression domain of shh in the pectoral fin bud of hmgb-knockdown embryos (J, red arrow), compared to the control fin bud (I, black arrow).

Expression Data

Genes:	GFP prrx1a ptch2 shha
Fish:	WT w25Tg WT + MO1-hmgb1a + MO1-hmgb2a + MO2-hmgb1a + MO2-hmgb2a w25Tg + MO1-hmgb1a + MO1-hmgb2a + MO2-hmgb1a + MO2-hmgb2a
Knockdown Reagents:	MO1-hmgb1a MO1-hmgb2a MO2-hmgb1a MO2-hmgb2a
Anatomical Term:	pectoral fin
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.