Fig. s2

miR-218 knockdown in zebrafish. (A) Schematic indicating a portion of the precursor sequences of the three miR-218 genes in zebrafish (miR-218a-1, miR-218a-2 and miR-218b). Sequences overlapping the region targeted by miR-218 MO¹ and miR-218 MO² that differ among miR-218 genes are indicated in blue. The location of the mature microRNA is indicated beneath and is also indicated in red. The Drosha cleavage site, which is targeted by the miR-218 MOs is indicated by an arrow, as is the Dicer cleavage site. Watson-Crick base-pairing is indicated by vertical lines, and colons indicate base-pairing that is not Watson-Crick for all of the miR-218 isoforms. (B) Knockdown of miR-218 was quantified by real-time qRT-PCR in control embryos or embryos injected with 5 or 10 ng of miR-218 MO². Expression of slit2 and slit3 was also monitored by real-time qRT-PCR. (C) The phenotype of embryos injected with miR-218 MO² is shown. miR-218 morphants have edema and circulation defects and minor vascular defects. Scale bar: 100 μm.