PUBLICATION
A Slit/miR-218/Robo regulatory loop is required during heart tube formation in zebrafish
- Authors
- Fish, J.E., Wythe, J.D., Xiao, T., Bruneau, B.G., Stainier, D.Y., Srivastava, D., and Woo, S.
- ID
- ZDB-PUB-110317-47
- Date
- 2011
- Source
- Development (Cambridge, England) 138(7): 1409-1419 (Journal)
- Registered Authors
- Stainier, Didier, Woo, Stephanie, Wythe, Joshua, Xiao, Tong
- Keywords
- Slit/Robo, Vegf, MicroRNA, Heart tube formation, Zebrafish
- MeSH Terms
-
- Analysis of Variance
- Animals
- Blotting, Western
- Cell Movement/physiology
- Cell Polarity/physiology
- Gene Expression Regulation, Developmental/physiology
- Glycoproteins/genetics
- Glycoproteins/metabolism*
- Heart/embryology*
- In Situ Hybridization
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Myocardium/metabolism*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Organogenesis/physiology*
- Receptors, Immunologic/genetics
- Receptors, Immunologic/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction/physiology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 21385766 Full text @ Development
Citation
Fish, J.E., Wythe, J.D., Xiao, T., Bruneau, B.G., Stainier, D.Y., Srivastava, D., and Woo, S. (2011) A Slit/miR-218/Robo regulatory loop is required during heart tube formation in zebrafish. Development (Cambridge, England). 138(7):1409-1419.
Abstract
Members of the Slit family of secreted ligands interact with Roundabout (Robo) receptors to provide guidance cues for many cell types. For example, Slit/Robo signaling elicits repulsion of axons during neural development, whereas in endothelial cells this pathway inhibits or promotes angiogenesis depending on the cellular context. Here, we show that miR-218 is intronically encoded in slit2 and slit3 and that it suppresses Robo1 and Robo2 expression. Our data indicate that miR-218 and multiple Slit/Robo signaling components are required for heart tube formation in zebrafish and that this network modulates the previously unappreciated function of Vegf signaling in this process. These findings suggest a new paradigm for microRNA-based control of ligand-receptor interactions and provide evidence for a novel signaling pathway regulating vertebrate heart tube assembly.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping