FIGURE

Fig. 2

ID

ZDB-FIG-110407-2

Publication

Padanad et al., 2011 - Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24

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Fig. 2

pax8 and sox3 interact in otic and epibranchial induction. (A–D) pax2a expression in the otic/epibranchial domain at 12 hpf in a control embryo (A), sox3 morphant (B), pax8 morphant (C) and sox3–pax8 double morphant (D). Numbers indicate normalized values for the mean ± standard deviation of the area of the pax2a expression domain (n = 10 specimens for each background). Area was calculated by outlining otic–epibranchial domains in Photoshop and measuring the number of pixels within. Differences between the morphants and the control were highly significant (p<.0005) as determined by t-tests. (E–H) Otic vesicles at 30 hpf in a live control embryo (E), sox3 morphant (F), pax8 morphant (G) and sox3–pax8 double morphant (H). (I–L) Expression of phox2a at 30 hpf in a control embryo (I), sox3 morphant (J), pax8 morphant (K) and sox3–pax8 double morphant (L). Positions of the facial ganglion (f) glossopharyngeal ganglion (g), and vagal ganglia (v₁ and v₂) are indicated. All images show lateral views with anterior to the left.

Expression Data

Genes:	pax2a phox2a
Fish:	AB AB + MO3-sox3 AB + MO6-pax8 + MO7-pax8 AB + MO3-sox3 + MO6-pax8 + MO7-pax8
Knockdown Reagents:	MO3-sox3 MO6-pax8 MO7-pax8
Anatomical Terms:	epibranchial field epibranchial ganglion otic placode
Stage Range:	5-9 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-sox3 AB + MO6-pax8 + MO7-pax8 AB + MO3-sox3 + MO6-pax8 + MO7-pax8
Knockdown Reagents:	MO3-sox3 MO6-pax8 MO7-pax8
Observed In:	epibranchial ganglion facial ganglion glossopharyngeal ganglion otic vesicle vagal ganglion 1 vagal ganglion 2 whole organism
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.