FIGURE

Fig. 2

ID

ZDB-FIG-110128-27

Publication

Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity

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Fig. 2

Organ laterality is randomized in setdb2-knockdown embryos. (A–L) Whole-mount mRNA in situ hybridization and statistical analyses of visceral and diencephalic laterality in the 5-MM control and setdb2 MO2-injected embryos at the indicated developmental stages with probes cmlc2 (A–C), lefty1 (D–F), cebpα (G–I, arrows), and insulin (J–L). (M–O) Determination of pancreatic laterality in 72-hpf Tg(insulin:EGFP) transgenic embryos injected with 5-MM control and setdb2 MO2. Dashed lines denote the embryonic midline. LV, liver; G, gut; L, Left side; R, Right side; C/B, central or bilateral; A, absent. All embryos are dorsal views with head to the top.

Expression Data

Genes:	cebpa EGFP ins lft1 myl7
Fish:	WT WT + MO2-setdb2 rj29Tg rj29Tg + MO2-setdb2
Knockdown Reagent:	MO2-setdb2
Anatomical Terms:	diencephalon gut heart tube liver pancreas
Stage Range:	20-25 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-setdb2
Knockdown Reagent:	MO2-setdb2
Observed In:	determination of digestive tract left/right asymmetry determination of heart left/right asymmetry determination of left/right asymmetry in diencephalon determination of liver left/right asymmetry determination of pancreatic left/right asymmetry diencephalon gut heart tube liver pancreas
Stage Range:	20-25 somites to Protruding-mouth

Phenotype Detail

Acknowledgments

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