FIGURE

Fig. S3

ID

ZDB-FIG-110113-10

Publication

Wang et al., 2011 - The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish

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Fig. S3

Mosaic expression of dominant-negative Rho kinase disrupts LR development. (A,B) The majority of embryos (93%, n=82) injected with 20 pg of control plasmid encoding yellow fluorescent protein (YFP) appeared normal at 2 dpf, including normal axis elongation (A). Most embryos injected with 20 pg of DNA encoding dominant-negative Rho kinase (DN-Rock) also showed normal axis elongation (75%, n=70), but some embryos developed mild (16%, n=70) or severe (9%, n=70) axial defects (B). (C,D) Asymmetric spaw expression in LPM was normal in the majority of control YFP DNA-injected embryos (C), but was altered in many DN-Rock embryos (see Fig. 4D), and was often bilateral (red arrows in D). Expression of shh in the midline was intact in uninjected embryos (n=94/94), control YFP DNA-injected embryos (n=59/59) and DN-Rock DNA-injected embryos (n=89/91). (E-H) Whereas heart looping was normal in most control embryos (E,H), DN-Rock embryos showed an increased frequency (H) of reversed (F) or no heart looping (G). Embryos with axial defects were excluded for this analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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