Fig. 2

Effect of inhibition of Cdc14B function on LR asymmetry. (A-L) In situ hybridization and expression pattern quantification in non-injected and cdc14b MO-injected zebrafish embryos of nkx2.5 (A-D), foxa3 (E-H) and spaw (I-L) at 31 hpf, 50 hpf and 19 hpf, respectively. (M) Quantification of LR asymmetry defects in non-injected and 0.5 ng or 1.5 ng MO1-cdc14b-injected wild-type and cdc14b^vu426 homozygous mutant embryos at 31 hpf using cmlc2:GFP transgene. (N) Rescue of the LR asymmetry phenotype in MO1-cdc14b-injected embryos. LR asymmetry defects were quantified using spaw expression pattern at 18 hpf. Numbers of embryos analyzed are indicated (n). Data are mean ± s.e.m. Statistics were carried out using Student′s t-test (D,H,L,M) and paired Student′s t-test (N), and are only presented for the left-sided heart class in M and the spaw left expression class in N.