FIGURE

Fig. 4

ID

ZDB-FIG-110112-12

Publication

Schwend et al., 2010 - Visualization of gli activity in craniofacial tissues of hedgehog-pathway reporter transgenic zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Reporter expression in Tg(Gli-d:mCherry) fish is sensitive to the Hh-signaling pathway.

(A–C) Lateral views, anterior to the left, of 30 hpf ethanol vehicle treated controls (left side of image) and Cya treated (right side) Tg(Gli-d:mCherry) siblings. (A,B) Live embryos were visualized using brightfield (A) and fluorescence (B) microscopy. 100 &mu:m Cya treated embryos showed a significant reduction in fluorescent mCherry protein when compared to their ethanol vehicle treated siblings. (C) Fixed embryos, stained with mCherry riboprobe revealed that 100 μm Cya treated embryos displayed significantly reduced mCherry RNA transcripts compared to their vehicle-treated siblings. (D) RT-PCR showing reduced gli1, and mCherry expression in 30 hpf embryos treated with 100 μm Cya compared to their nontreated (NT) siblings. Two loading controls for disp1 and gapdh were included. (E–H) Lateral views of 30 hpf noninjected controls (E,F) or Tg(Gli-d:mcherry) siblings injected with 50 pg shh at the one cell stage (G,H). Siblings injected with shh mRNA showed expanded reporter expression throughout the trunk when compared to noninjected controls.

Expression Data

Gene:	gli1
Fish:	nu15Tg
Anatomical Term:	whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One