FIGURE

Fig. 4

ID

ZDB-FIG-110105-39

Publication

Hans et al., 2011 - Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Cre-mediated recombination of the (hsp70l:mCherry-T2A-CreER^T2)^#152 allele in the red-to-green reporter line in the absence and presence of heat and TAM. A: Scheme of the transgene combination of the embryos depicted in B–E. B: In the absence of heat and TAM, the combination of the Tg(hsp70l:mCherry-T2A-CreER^T2)^#15 allele with the red-to-green reporter line results in strong DsRed2 but no EGFP expression in double transgenic embryos. C: Application of TAM leads to EGFP expression in the Tg(hsp70l:mCherry-T2A-CreER^T2)^#15 enhancer trap as well as a mosaic pattern in double transgenic embryos at permissive temperatures. D: In the absence of TAM, mosaic EGFP expression can be detected after heat treatment. E: Application of TAM and heat results in strong ubiquitous EGFP expression in double transgenic embryos. B–E: Lateral views of live 24-hpf embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.