FIGURE

Fig. 2

ID

ZDB-FIG-101206-18

Publication

den Hollander et al., 2010 - Genetic defects of GDF6 in the zebrafish out of sight mutant and in human eye developmental anomalies

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Fig. 2

Positional cloning of the zebrafish out of sight mutation. (A) The out locus maps to chromosome 16 between markers Z6293 and Z8819. Sequence analysis of the gdf6a gene from this interval identified a mutation affecting its start codon (c.1A > G, p. Met1Val) in mutant (C) but not wild-type (B) individuals. SP = signal peptide, ASD = active signaling domain. (D, E) The overexpression of wild-type (E) but not mutant (D) gdf6a causes ventral eye defects. Shown are lateral (left panels) and ventro-lateral (right panels) views of zebrafish larvae at 3 dpf. Anterior is to the left, asterisks indicate the lens. (F) The frequency of ventral eye defects, following the overexpression of wild-type or mutant gdf6a in four independent experiments, as indicated on the horizontal axis (1 through 4). Numbers above the bar graph indicate sample sizes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gdf6a^m233/m233 (AB)
Observed In:	eye photoreceptor cell retinal ganglion cell layer cell retinal inner nuclear layer cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genet.