FIGURE

Fig. 1

ID

ZDB-FIG-101122-12

Publication

Appelbaum et al., 2009 - Sleep-wake regulation and hypocretin-melatonin interaction in zebrafish

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Fig. 1

Zebrafish HCRT neurons are glutamatergic. (A–D) and close-ups (A1–D1) Double fluorescent ISH between hcrt mRNA and fast neurotransmitter phenotype markers as visualized using confocal microscopy on adult brain sections (reconstructed stacks of 0.5- or 1-μm sections). (C2–C4) and (D2–D4): single-plane, high-magnification pictures of hcrt cells (green, C2, D2), vglut2a or -b cells (red, C3, D3), and merged views (C4, D4). Arrowheads indicate cells coexpressing hcrt and vglut2a or vglut2b. Note the frequent colocalization. Absence of coexpression is occasionally observed (arrow). [Scale bar, 100 μm (A–D), 20 μm (A1–D1, C2–C4, and D2–D4).]

Expression Data

Genes:	gad1b hcrt slc17a6a slc17a6b slc17a7a
Fish:	WT
Anatomical Terms:	brain glutamatergic neuron brain neuron
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA