Fig. S1
Increased cell death in the eyes of radars327 mutants. Lateral views of whole-mount TUNEL-stained embryos. (A) 24 hours post-fertilization (hpf) WT embryos have small numbers of TUNEL-positive cells throughout the head and trunk. (B) 24hpf radars327 mutant embryos have a large increase in TUNEL reactivity in the developing eye. (C and D) 30 hpf radars327 mutant embryos have significantly more TUNEL-positive cells in the eyes than WT. The difference is less dramatic in the trunk. (E) Quantification of TUNEL staining in 30 hpf embryos. radars327 mutants (n = 6) have significantly more TUNEL-positive cells in the eye than WT embryos (n = 8, t test, P < 0.000001). |