Fig. 7

pH 3/DAPI staining of wild-type, shh^-/- and p53^-/-shh^-/- retinas was done at 24 hpf (A), 34 hpf (B) and 48 hpf (C). At all stages, the mitotic index in the shh^-/- retina was lower than in the wild-type retina, while in the p53^-/-shh^-/- retina, the mitotic index was comparable to that of the wild-type retina. (D) Statistical analysis of mitotic indices in wild-type, p53^-/-, shh^-/- and p53^-/-shh^-/- retinas at 24 hpf, the indices being comparable in wild-type (14,9%, SD = 2,4%), p53^-/- (15,3%, SD = 2,4%), and p53^-/-shh^-/- (13,1%, SD = 1,9%) embryos and significantly higher than in the shh^-/- mutant retina (7,5%, SD = 2,3%). (E) Statistical analysis of mitotic indices in the wild-type, p53^-/-, shh^-/- and p53^-/-shh^-/- retinas at 34 hpf, comparable in wild-type (11,8%, SD = 1,73%), p53^-/- (12,02%, SD = 1,46%), and p53^-/-shh^-/- (12,1%, SD = 1,9%) retinas, whereas the mitotic index in the shh^-/- mutant retina was almost twice lower (7%, SD = 1,38%). (F) Statistical analysis of mitotic indices in wild-type, shh^-/- and p53^-/-shh^-/- retinas at 48 hpf, the indices being comparable in wild-type (11,1%, SD = 2%) and p53^-/-shh^-/- (11,6%, SD = 1,54%) embryos and significantly higher than in the shh^-/- mutant retina (7,16%, SD = 1,25%). Either 8 (D) or 10 (E, F) embryos (2 retinal sections per embryo) were analysed for each genotype and mean +/- standard deviation indicated above the bars of mitotic index statistics. Asterisks (***) on top of shh^-/- mutant bars indicate their significant statistical differences from other samples (t-test, P-value<0,001).