Fig. S4

Expression of hoxc10a and DiI-labeling in zebrafish embryos injected with gdf11 MO. (A) Measurement of the anterior border of hoxc10a expression in the spinal cord in gdf11 morphants. Note that the anterior border of hoxc10a expression was in somite 15 in most gdf11 morphants (26/27 in 100ng gdf11 morphants; 29/35 in 40ng gdf11 morphants). (B-D) Cell lineage analysis in gdf11 morphants. Locations of DiI-labeled cells are indicated by arrowheads. (B) Schematic diagram showing the location of DiI (red) application. (B′) DiI was applied to the lateral plate mesoderm cells opposite somite 15 in 16 hpf zebrafish gdf11 morphants. (C, D) Schematic diagrams of zebrafish embryos at 36 hpf (C) and 6 dpf (D). (C′, D′) Higher magnification of the labeled regions shown in (C) and (D), respectively. Scale bars: 100 μm. (E) Cell lineage analysis in zebrafish gdf11 morphants. Numbers above each column indicate the opposite somite levels. The purple bar indicates hoxc10a expression in the spinal cord at 16 hpf of gdf11 morphants. Sample numbers are shown at left. Note that the DiI-labeled cells within somite 15, where the anterior border of hoxc10a expression is observed in the spinal cord of gdf11 morphants at 16 hpf, were identified in the pelvic fin position of gdf11 morphants (somite 9-10 levels) by 36 hpf, and continued to be in the same position at 6 dpf.