FIGURE

Fig. S9

ID

ZDB-FIG-101008-12

Publication

Imai et al., 2010 - The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish

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Fig. S9

In the fzr1 morphant, proliferation of lens epithelial cells is affected, but specification of lens epithelium and lens fiber cells occur at 48 hpf. (A-C′′) Labeling of 48 hpf fzr1 morphant lens with anti-AQP0 (A), anti-Pax6 (B) and anti-pH3 (C) antibodies. Nuclei were labeled with DAPI (A′-C′); merge (A′′-C′′) AQP0 and Pax6 were expressed in lens fiber cells and lens epithelial cells, respectively. pH3-positive epithelial cells were not increased in number (arrowhead in C-C′′), suggesting that cell cycle arrest in M phase is unlikely in the fzr1 morphant. (D-E′′) BrdU incorporation of fzr1 morphant at 48 hpf (D) and 72 hpf (E). Nuclei are labeled with DAPI (D′,E′); merge (D′′,E′′). No BrdU incorporation was detected in lens epithelium in fzr1 morphant at both stages, suggesting that cell proliferation is affected in the fzr1 morphant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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