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Fig. S9 In the fzr1 morphant, proliferation of lens epithelial cells is affected, but specification of lens epithelium and lens fiber cells occur at 48 hpf. (A-C′′) Labeling of 48 hpf fzr1 morphant lens with anti-AQP0 (A), anti-Pax6 (B) and anti-pH3 (C) antibodies. Nuclei were labeled with DAPI (A′-C′); merge (A′′-C′′) AQP0 and Pax6 were expressed in lens fiber cells and lens epithelial cells, respectively. pH3-positive epithelial cells were not increased in number (arrowhead in C-C′′), suggesting that cell cycle arrest in M phase is unlikely in the fzr1 morphant. (D-E′′) BrdU incorporation of fzr1 morphant at 48 hpf (D) and 72 hpf (E). Nuclei are labeled with DAPI (D′,E′); merge (D′′,E′′). No BrdU incorporation was detected in lens epithelium in fzr1 morphant at both stages, suggesting that cell proliferation is affected in the fzr1 morphant.