FIGURE

Fig. 3

ID

ZDB-FIG-100913-6

Publication

May-Simera et al., 2010 - Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish

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Fig. 3

Reduced expression of bbs8 and vangl2/tri results in laterality defects. Cardiac looping and the laterality marker expression. A-D) Cardiac looping at 30 hfp. A) Normal looping (D-loop). B) Reversed looping (L-loop). C) Absent looping. The heart tube is highlighted by a red dotted line (A-C). D) At 30 hpf, injected tri^-/- embryos exhibited increased percentages of abnormal cardiac looping. The fractions of embryos of each category are noted in percent along the X-axis. E-G) spaw expression (arrowheads) in 15-16-somite embryos. E) Normal spaw expression on the left lateral mesoderm. F) Bilateral and G) inverted spaw expressions. H) Quantitative representation of spaw expression. In tri embryos injected with bbs8-MO, the expression is randomised. Views: ventral (A-C), dorsal (E-G).

Expression Data

Gene:	spaw
Fish:	TL
Anatomical Term:	lateral plate mesoderm left side
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vangl2^m209/m209 TL + MO3-ttc8 vangl2^m209/m209 + MO3-ttc8
Knockdown Reagent:	MO3-ttc8
Observed In:	embryonic heart tube left/right pattern formation heart looping heart tube left/right pattern formation
Stage Range:	14-19 somites to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 345(2), May-Simera, H.L., Kai, M., Hernandez, V., Osborn, D.P., Tada, M., and Beales, P.L., Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish, 215-225, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.